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opti-mem reduced serum media  (Thermo Fisher)


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    Structured Review

    Thermo Fisher opti-mem reduced serum media
    Opti Mem Reduced Serum Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti-mem+reduced+serum+media/opti+mem+i+reduced+serum+medium/pmc10515159__pnas__2218150120__sapp-7-47-51
    Average 90 stars, based on 1 article reviews
    opti-mem reduced serum media - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Knockdown:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Transfection:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Control:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Sequencing:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Virus:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Plasmid Preparation:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Recombinant:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.

    Software:

    Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage
    Article Snippet: Opti-MEM TM reduced serum media , Thermo Fisher , Cat#31985062.

    Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells
    Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Opti-MEM reduced serum media (Thermo Fisher) according to the manufacturer's instructions.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB.
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNAVPXsiv, and 45 μg PEI.

    Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration.
    Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Opti-MEM reduced serum media (Thermo Fisher Scientific) following the manufacturer’s instructions.

    Article Title:
    Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Opti-MEM reduced serum media (Gibco) for 8 hours.

    Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB
    Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Opti-MEM Reduced Serum Media (Thermo Fisher Scientific), 12 μg of gGn-p6* virus plasmid, 3 μg pcCNA-VPXsiv, and 45 μg PEI.

    Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes.
    Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Opti-MEM Reduced Serum Media (Thermo Fisher Scientific).

    Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces
    Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Opti-MEM reduced serum media (Thermo Fisher Scientific, catalog no. 31985062) at 37°C overnight.



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