opti-mem reduced serum media (Thermo Fisher)
90
Structured Review
Thermo Fisher
opti-mem reduced serum media
Opti Mem Reduced Serum Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opti-mem+reduced+serum+media/opti+mem+i+reduced+serum+medium/pmc10515159__pnas__2218150120__sapp-7-47-51
Average 90 stars, based on 1 article reviews
Opti Mem Reduced Serum Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opti-mem+reduced+serum+media/opti+mem+i+reduced+serum+medium/pmc10515159__pnas__2218150120__sapp-7-47-51
Average 90 stars, based on 1 article reviews
opti-mem reduced serum media - by Bioz Stars,
2026-10
90/100 stars
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Cell Culture:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Knockdown:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Transfection:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Control:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Sequencing:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Virus:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Plasmid Preparation:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Recombinant:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in Software:Article Title: Structural development of the HIV-1 apex-directed PGT145-PGDM1400 antibody lineage Article Snippet: Article Title: Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cells Article Snippet: Reverse transfection was performed on INS 832/13 or dispersed rat islet cells using Lipofectamine 2000 and Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB. Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration. Article Snippet: Cells were never exposed to direct light during handling and were cultured in an incubator with a humidified atmosphere, 5% CO2, and 37 C. For light-exposure experiments, the medium was replaced by serum-free high-glucose DMEM just before light exposure to sensitize cells to light-induced damage, as previously reported.21 All reverse transfections in 661W cells were performed with INTERFERin transfection reagent (Polyplus) mixed with Article Title: Article Snippet: Unless otherwise stated, all cell culture experiments were performed in biological triplicate, meaning separate cell expansion and collection. siRNA Knockdown HUVEC were transfected at ~ 80% confluency using a control sequence or siRNAs targeted against human PDHA1, LONP1, ACLY, SLC25A1, or RELA with lipofectamine RNAiMAX (Thermo) in Article Title: GS143, an inhibitor of E3 ligase β-TrCP, reverses HIV-1 latency without activating T cells via unconventional activation of NFκB Article Snippet: The next day, transfection mix was prepared for each dish using 1 mL Article Title: Integrative miRNOMe profiling reveals the miR-195-5p-CHEK1 axis and its impact on luminal breast cancer outcomes. Article Snippet: Working solutions at lower concentrations were freshly prepared by diluting the respective stock solutions in the Article Title: Vibrio MARTX toxin binding of biantennary N-glycans at host cell surfaces Article Snippet: Kifunensine (R&D Systems, catalog no. 3207) was applied to cultured cells at 0.02 mM in |